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KMID : 0368420170600050472
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Journal of Plant Biology
2017 Volume.60 No. 5 p.472 ~ p.484
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Genome-wide characterization and stress-responsive expression profiling of MCM genes in Brassica oleracea and Brassica rapa
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Shanmugam Ashokraj
Robin Arif Hasan Khan
Thamilarasan Senthil Kumar
Vijayakumar Harshavardhanan
Natarajan Sathishkumar
Kim Hoy-Taek
Park Jong-In
Nou Ill-Sup
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Abstract
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The Minichromosome maintenance protein [MCM (2-7)] complex is associated with helicase activity for replication fork formation during DNA replication. We identified and characterized each 12 putative MCM genes from Brassica oleracea and Brassica rapa. MCM genes were classified into nine groups according to their evolutionary relationships. A high number of syntenic regions were present on chromosomes C03 and A03 in B. oleracea and B. rapa, respectively, compared to the other chromosomes. Expression analysis showed that most of the MCM(2-7) helicase-subunit genes and their coregulating MCM genes were upregulated during hydroxyurea (HU) induced stress in B. oleracea. In B. rapa, MCM(2-7) helicase genes BrMCM2_2, BrMCM7_1, BrMCM7_2 and their co-regulating genes were upregulated during replication stress. During cold stress, BoMCM6 in B. oleracea and BrMCM5 in B. rapa were remarkably upregulated. During salt stress, BoMCM6_2, BoMCM7_1, BoMCM8, BoMCM9, and BoMCM10 were markedly upregulated in B. oleracea. Hence, our study identified the candidate MCM family genes those possess abiotic stress-responsive behavior and DNA replication stress tolerance. As the first genome-wide analysis of MCM genes in B. oleracea and B. rapa, this work provides a foundation to develop stress responsive plants. Further functional and molecular studies on MCM genes will be helpful to enhance stress tolerance in plants.
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KEYWORD
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Abiotic stress, Brassica crops, Hydroxyurea (HU), Phylogenetic tree, Replication stress, Structure of MCM(2-7)
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